Target clones identification

Artikelnummer:PHASE 3
Menge:
Technische Daten
Beschreibung

Isolation of rabbit PBMCs (peripheral blood mononuclear cells) from rabbit with the best immune response. Enrichment of immunogen specific b cells / plasmablasts. Amplification of cognate pair of heavy and light chain variable regions by emulsion single cell RT-PCR. NGS analysis of cognate pairs (> 2mio. paired end reads). Bioinformatic evaluation of sequencing reads and determination of clonal distribution. Deliverables: DNA sequencing data of variable regions of 10 enriched clones.

Literatur

Kontakt