CD15 Granulocytes

TÜ9, Ms, 1 ml RTU

Artikelnummer:2-CD072-07
Menge:
Technische Daten
Reaktivität AK:Human, others not tested.
Format AK:Ready-to-use
Klon:TÜ9
Spezies:Mouse
Ig Unterklasse:IgM
Immunogen:Cells of an acute myelomonocyte leucaemia
Anwendung AK:IHC(p),IHC(f)
Fixierung:1) NOTOXhisto 2) Formalin
Zelluläre Lokalisation:Cytoplasmic, membranous
Kontrolle:Hodgkin's lymphoma
Lagerung AK:Store at 2 - 8 °C. Do not use after expiration date indicated on the vial.
Synonyme:3-fucosyl-N-acetyl-lactosamine, Lewis x, SSEA-1, sialyl-Lewis X, 3-fucosyl- acetyllactoseamine
Verfügbar in folgenden Ländern:world wide (except US)
Status:IVD
Beschreibung

TÜ9 belongs to the CD15 group of antibodies and recognizes the epitope 3-fucosyl-N-Acetyl-lactosamine of an antigen which is present on myeloid cells, mainly on granulocytes. TÜ9 is reactive with tumor cells (Hodgkin and Sternberg-Reed cells) in biopsy material from most patients suffering from Hodgkins disease. It is one of the most valuable and reliable markers for Hodgkins disease providing a typical membrane and cytoplasmic „dot-like“ staining in the majority of Sternberg-Reed cells and their variants. This antibody is not expressed by T- cells, B-cells, monocytes, erythrocytes and platelets.

Literatur

[1] Angel CA, Warford A, Day SJ & Lauder I (1989): Comparative quality assessment in immunocytochemistry: pilot study of CD15 staining in paraffin wax embedded tissue in Hodgkin's disease. J Clin Pathol. 42(10): 1096-100.
[2] Nakayama F, Nishihara S, Iwasaki H, et al. (2001): CD15 expression in mature granulocytes is determined by alpha 1,3-fucosyltransferase IX, but in promyelocytes and monocytes by alpha 1,3-fucosyltransferase IV. J Biol Chem. 276(19): 16100-6.
[3] Pinkus GS, Thomas P & Said JW (1985): Leu-M1--a marker for Reed-Sternberg cells in Hodgkin's disease. An immunoperoxidase study of paraffin-embedded tissues. Am J Pathol. 119(2): 244-52.
[4] Stein H, Gerdes J, Schwab U, et al. (1982): Identification of Hodgkin and Sternberg-reed cells as a unique cell type derived from a newly-detected small-cell population. Int J Cancer. 30(4): 445-59.
[5] Stein H, Uch‡nska-Ziegler B, Gerdes J, et al. (1982): Hodgkin and Sternberg-Reed cells contain antigens specific to late cells of granulopoiesis. Int J Cancer. 29(3): 283-90.
[6] Stein H, Gerdes J, Schwab U, et al. (1983): Evidence for the detection of the normal counterpart of Hodgkin and Sternberg-Reed cells. Hematol Oncol. 1(1): 21-9.
[7] Stein H, Gerdes J, Schwab U, et al. (1983): Evidence for the origin of Hodgkin and Sternberg-Reed cells from a newly detected small cell population. Haematol Blood Transfus. 28: 407-10.
[8] Guzman J, Bross KJ & Costabel U (1991): Malignant lymphoma in pleural effusions: an immunocytochemical cell surface analysis. Diagn Cytopathol. 7(2): 113-8.
[9] Niedieck B & Löhler J (1987): Expression of 3-fucosyl- N-acetyllactosamine on glia cells and its putative role in cell adhesion. Acta Neuropathol. 75(2): 173-84.

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